Design and methodology of multiparametric phase mapping of microscopic images of rat blood films in the differential diagnosis of sepsis severity

Authors

DOI:

https://doi.org/10.31649/1681-7893-2026-51-1-160-167

Keywords:

phase mapping, sepsis, polarization microscopy, statistical analysis, biological tissues

Abstract

The paper addresses the problem of differential diagnosis of sepsis severity based on microscopic images of blood films. A multiparametric phase mapping method based on statistical analysis of phase shift distributions is proposed. It is experimentally shown that with increasing pathology severity, the mean and variance decrease, while skewness and kurtosis increase. The results confirm the effectiveness of the method for quantitative assessment of pathological states. The evaluation of the effectiveness of the method showed a high level of differentiation between groups. In particular, for the parameters of asymmetry and kurtosis, excellent diagnostic quality was achieved (over 90% balanced accuracy), while for the mean and variance, good and satisfactory indicators were obtained depending on the groups being compared. This confirms the high informativeness of the statistical parameters of phase maps as diagnostic criteria. The obtained results confirm the effectiveness of the polarization-phase mapping method for objective differential diagnosis of the severity of sepsis and demonstrate its sensitivity to structural changes in biological environments

Author Biographies

O.G. Ushenko, Yuriy Fedkovych Chernivtsi National University

Доктор фізико-математичних наук, професор, завідувач кафедри поліграфічних, мультимедійних та оптичних технологій

O.V. Dubolazov, Yuriy Fedkovych Chernivtsi National University

Доктор фізико-математичних наук, професор кафедри поліграфічних, мультимедійних та оптичних технологій

I.G. Kurek, Yuriy Fedkovych Chernivtsi National University

Кандидат фізико-математичних наук, молодший науковий співробітник  кафедри комп’ютерних наук

O.V. Olar, Yuriy Fedkovych Chernivtsi National University

Кандидат фізико-математичних наук, асистент кафедри комп’ютерних наук

A.Yu. Volovyk, Vinnytsia National Technical University

Доктор технічних наук, доцент, професор кафедри інформаційних радіоелектронних технологій і систем

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Ushenko O, Bilookyi O, Zheng J, Dubolazov A, Olar O, Ushenko Y, et al. 3D digital holographic polarimetry. Front Phys. 2024;12:1426469. https://doi.org/10.3389/fphy.2024.1426469

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Published

2026-06-17

How to Cite

[1]
O. Ushenko, O. Dubolazov, I. Kurek, O. Olar, and A. . Volovyk, “Design and methodology of multiparametric phase mapping of microscopic images of rat blood films in the differential diagnosis of sepsis severity”, Опт-ел. інф-енерг. техн., vol. 51, no. 1, pp. 160–167, Jun. 2026.

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Biomedical Optical And Electronic Systems And Devices

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